An Easy Method for Agrobacterium tumefaciens-Mediated Gene Transfer to Nicotiana tabacum cv. TAPM26
نویسندگان
چکیده
منابع مشابه
High-Efficiency Agrobacterium-Mediated Transformation of Tobacco (Nicotiana tabacum)
To improve Agrobacterium-mediated transformation of tobacco, factors influencing gene delivery, including genotype of the plant, bacterial strain, and Agrobacterium transformation procedure, were tested via direct somatic embryogenesis. Leaf tissue of three different tobacco genotypes (Nicotiana tabacum L. cvs. Samsun, and Xanthi, and N. benthamiana) we...
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. ...vii Chapter 1. Introduction . . .....1 Chapter 2. Review of Literature ... . . 3 2.1 Molecular Mechanism of Agrobacterium-mediated DNA Transfer ........... 3 2.2 Adoption for Plant Molecular Biology . ...10 2.3 Agrobacterium-mediated Plant Transformation Protocol Development . ...12 Chapter 3. Mate...
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Abiotic stress conditions can be imposed on plants through either poor water quality or adverse climate condition. These types of stress affect plant growth and development throughout their life cycle. Plants that are tolerant to drought are therefore valuable, and turf grasses have the potential to grow tolerant. Turf grasses provide ground cover and offer multiple benefits, from their aesthet...
متن کامل1-Aminocyclopropane-1-carboxylate deaminase enhances Agrobacterium tumefaciens-mediated gene transfer into plant cells.
Agrobacterium-mediated gene transfer is widely used for plant molecular genetics, and efficient techniques are required. Recent studies show that ethylene inhibits the gene transfer. To suppress ethylene evolution, we introduced 1-aminocyclopropane-1-carboxylate (ACC) deaminase into Agrobacterium tumefaciens. The ACC deaminase enhanced A. tumefaciens-mediated gene transfer into plants.
متن کاملNucleotide Sequence of the Nicotiana tabacum cv Xanthi Gene Encoding 1-Aminocyclopropane-1-Carboxylate Synthase.
Ethylene is a plant hormone that influences many aspects of plant growth and development (10). The biosynthetic pathway of ethylene production in higher plants is well defined. The rate-limiting step in this pathway is the reaction catalyzed by ACC' synthase (S-adenosyl-L-methionine methyl-thioadenosine-lyase, EC 4.4.1.4), which converts Sadenosyl-L-methionine to ACC. Tobacco (Nicotiana tabacum...
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ژورنال
عنوان ژورنال: Journal of Biological Sciences
سال: 2010
ISSN: 1727-3048
DOI: 10.3923/jbs.2010.480.489